Adsorption of thiocyanate by anion-exchange resins and its analytical application.

al.(12), I did not find the technique 12. Merlini J, Piro P, Pavesi F, et al. weakly basic resin Amberlite IRA-68 susceptible to technical artefacts. Detection and identification of monoclo(Rohm and Haas), which has a polyImmunofixation on cellulose acenal components: Immunoelectrophoreacrylic matrix, has practically no affintate deserves wider use for identifisis on agarose gel and immunofixation ity for thiocyanate under the condication of monoclonal gammopathies on cellulose acetate compared. C/in tions of our assay. The same was true in the clinical laboratory. Chem 27, 1862-1865 (1981). for Amberlite IRC-50 (Rohm and Haas), a weakly acid cation-exchange John T. Whicher References resin with a polyacrylic matrix. A polystyrene resin without ion exchange groups (“Bio Beads SM 2,” Bio-Rad Laboratories) has a small but significant affinity for thiocyanate. In contrast, the strongly basic anion-exchange resins Amberlite IRA-400 and Amberlite IRA-410 (Rohm and Haas) bind thiocyanate so strongly, presumably by electrostatic forces, that they are unsuitable for use in our thiocyanate assay. In conclusion: thiocyanate is bound to certain weakly basic anion-exchange resins with a polystyrene matrix at a high pH, where an ion-exchange mechanism is impossible. This effect may be used for analytical purposes and illustrates the possible importance of the matrix for separations on ion-exchangeresins.Consequently, manufacturers of such resinsshould not change their manufacturing procedures without notice to their customers, even if the changes do not involve the functional groups of the resin. Dept. of Chem. Pat/wi. Bristol Royal Infirmary Bristol BS2 8HW UK.